Planta Med. (2005) Vol. 71. P. 446-451.
Caffeic Acid Metabolites from Eritrichium sericeum Cell
Cultures
Fedoreyev S.A., Veselova M.V., Krivoschekova O.E., Mischenko N.P., Denisenko
V.A., Dmitrenok P.S., Glazunov V.P., Bulgakov V.P., Tchernoded G.K., Zhuravlev
Y.N.
Correspondence: Dr. Sergey A. Fedoreyev Pacific Institute of Bioorganic Chemistry
of the Far East Branch of Russian Academy of Sciences Vladivostok, 690022,
Prospect 100 let Vladivostoku, 159 Russian Federation
Fax: 4232 314050 E-mail: fedoreyev@mail.primorye.ru
Abstract
Eritrichium sericeum (Boraginaceae) callus and root cultures were
established and analyzed for caffeic acid metabolite (CAM) production. Two substances,
(-)-rabdosiin and rosmarinic acid were identified as main CAMs produced by these
cultures. The E. sericeum Er-1 root culture accumulated up to 1.5% and
4.5% DW of (-)-rabdosiin and rosmarinic acid, respectively. Rabdosiin in the
L. erythrorhizon callus cultures was produced exclusively as (+)-enantiomer
while in both Eritrichium cultures as (-)-enantiomer. The E. sericeum
Er-1 culture accumulated 3-fold higher levels of CAMs than the L. erythrorhizon
culture. A new compound, named eritrichin, was isolated from the cultured E.
sericeum cells. The structure of this compound was established as (2R)-3-(3,4-dihydroxyphenyl)-2-{[4-(3,4-dihydroxyphenyl)-6,7-dihydroxy-2-naphthoil]oxy}propanoic
acid on the basis of spectral data.